Textbook in PDF format

This volume explores the latest ways to detect hypoxia in the context of cancer, including techniques to study gene expression changes, protein responses, and cellular adaptations to low-oxygen conditions. This book also covers new protocols to characterize hypoxia in tumors with high spatial resolution and novel therapeutic approaches that consider the complex microenvironments of solid tumors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Hypoxia: Methods and Protocols is a valuable tool to help cancer researchers and other scientists detect hypoxia in cancer and other disease pathologies.
Preface
Contributors
Fluorescent Reporter Methods for Hypoxia Detection
Genetically Encoded Reporters to Monitor Hypoxia
Detection of Hypoxia in 2D and 3D Cell Culture Systems Using Genetically Encoded Fluorescent Hypoxia Sensors
Mapping the Fate of Hypoxic Cells Using an Irreversible Fluorescent Switch
Application of a Specific and Sensitive NQO1 Turn-On Near-Infrared Fluorescence Probe for Live Cancer Cell and Xeno...
Non-fluorescent Reporter Methods for Hypoxia Detection
A Luciferase Reporter Assay to Detect Cellular Hypoxia In Vitro
Oxygen Assessment in Tumors In Vivo Using Phosphorescence Lifetime Imaging Microscopy
Radionuclide Reporter Imaging to Visualize Tumor Hypoxia Ex Vivo and In Vivo
Measuring Pericellular Oxygen Tension for In Vitro Cell Culture
A Review of Hypoxia Imaging Using 18F-Fluoromisonidazole Positron Emission Tomography
Photoacoustic Lifetime Imaging of Hypoxia
Staining Hypoxic Areas of Frozen and FFPE Tissue Sections with Hypoxyprobe
Methods to Detect Hypoxia Inducible Factors and their Downstream Targets
Multiplex Immunofluorescence Staining Protocol for the Dual Imaging of Hypoxia-Inducible Factors 1 and 2 on Formal...
Detecting Hypoxia-Inducible Factor Levels and Activity
Methods to Detect Hypoxia-Induced Changes in Cell Metabolism
Metabolomic Investigations into Hypoxia-Mediated Metabolic Reprogramming of Pancreatic Cancer Cells
Measurement of Metabolic Alteration in Immune Cells Under Hypoxia
Methods to Simulate Hypoxia, Evaluate Oxygen Consumption Rates, and Measure FIH Activity Levels
Evaluation of Oxygen Consumption Rates In Situ
Cleanroom-Free Microfluidic Device for Natural Induction of Hypoxia in 2D and 3D Tumor Models
Oxomer- and Reporter Gene-Based Analysis of FIH Activity in Cells
Index